DIRECT TESTING OF ANTIBIOTIC SENSITIVITY BY A MICROCOLONY METHOD

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Abstract
A microcolony method of measuring antibiotic sensitivity requiring 4 hours incubation, was adapted to testing of organisms obtained directly from swabs, urines, and blood cultures. The results of the "direct" 4-hour test were compared with those obtained by prolonging incubation to 20 hours and with results obtained by carrying Out similar tests on the individual organisms isolated in pure culture ("indirect" tests). All of 12 urine samples and 6 infected blood specimens proved suitable for testing but only 35 of 68 swabs were suitable. Unequivocal results in direct tests were obtained in 94.3% of 4-hour tests and 95% of 20-hour tests. The result of the 4-hour direct test differed from that of the 20-hour direct test on 2.8% of occasions, and from those of the 4-hour indirect and the 20-hour indirect test on more than 5% of occasions. Discrepancies occurred about twice as often with staphylococci as with enterobacteria.

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