Staphylococcal Enterotoxin B Thermal Inactivation in Milk

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Abstract
The times and temperatures required to inactivate 30 [mu]g/ml of purified enterotoxin B in raw milk were determined using a double-gel-diffusion assay procedure with a sensitivity of 0.5 [mu]g/ml. With heating temperatures of 210, 220, 230, 240, 250, and 260[degree]F, enterotoxin inactivation required 134.2, 90.5, 51.2, 32.6, 18.4, and 12.1 min. respectively. The D values ranged from 68.5 to 6.2 min. and the z value for purified enterotoxin B in milk was 46.6[degree]F. The experimental activation energy was 25,900 cal/g mole; standard enthalpy of activation at 120[degree]C was 25,100 cal/g mole; standard entropy of activation at 120[degree]C was -8.1 cal/g moke K; and the standard free energy of activation at 120[degree]C was 28,300 cal/g mole.