The characterisation of the shikimate pathway enzyme dehydroquinase fromPisum sativum
- 8 August 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 349 (3) , 397-402
- https://doi.org/10.1016/0014-5793(94)00710-1
Abstract
Peptides accounting for 157 residues of the bifunctional shikimate pathway enzyme, dehydroquinase/shikimate dehydrogenase, ofPisum sativumwere sequenced. Three of the peptides were homologous to regions inEscherichia colidehydroquinase and two toE. colishikimate dehydrogenase. The pea dehydroquinase activity was inhibited by treatment with dehydroquinate plus sodium borohydride, establishing it as a type I dehydroquinase. Synthetic oligonucleotides designed from the amino acid sequence were used as PCR primers to amplify fragments ofP. sativumcDNA. DNA sequence analysis showed that these amplified products were derived from dehydroquinase/shikimate dehydrogenase CDNA. The complete amino acid sequence of the dehydroquinase domain has been defined; it is homologous to all other type I dehydroquinases and is N‐terminal.Keywords
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