Temperature-dependent31P n.m.r. shifts to assess the conformations of branched tri- and tetra-nucleotides which are formed as lariats in the splicing reactions
- 1 January 1988
- journal article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Chemical Communications
- No. 8,p. 542-544
- https://doi.org/10.1039/c39880000542
Abstract
Temperature-dependent changes of 31P n.m.r. chemical shifts (Δδ) have been used to estimate the secondary structure of branched tri- and tetra-nucleotides formed in the splicing reaction; these studies have provided independent evidence, confirming our earlier 1H n.m.r. studies, that the 2′→ 5′ stacking is the predominant structural feature that dictates the secondary structure of the branched trinucleotides while the branched tetranucleotides exist in the form of a distorted A-RNA helix without any 2′→ 5′ stacking.Keywords
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