Characteristic differences in inhibitory effects of oligodeoxyribonucleotides from DNA on human systemic lupus erythematodes (SLE) sera and rabbit anti-DNA sera.

Publisher Website
Google Scholar
Abstract
The difference in the antigenic determinant size of DNA for sera from patients with SLE and rabbit anti-DNA sera were investigated. Haptenic inhibition studies were done by measuring the inhibition of [3H]DNA-antibody binding by 3 different types of oligonucleotides which were prepared from formic acid-diphenylamine digests, hydrazinolyzed digests and pancreatic DNase digests, respectively. Oligonucleotides from DNase I digests showed potent inhibitory activity with SLE sera and rabbit sera. The inhibitory activities of purine and pyrimidine oligonucleotides were more potent for SLE sera than for rabbit anti-DNA sera. The determinant size estimated for rabbit sera was in the range of tetra- to heptanucleotide, while in SLE sera it was in the range of the di- and trinucleotide.
Keywords

This publication has 0 references indexed in Scilit: