Is the Cell Membrane a Universal Rate-Limiting Barrier to the Movement of Water between the Living Cell and Its Surrounding Medium?

Abstract
With the use of a newly introduced technique, the "influx profile analysis," we studied the diffusion of tritiated water in and out of frog ovarian eggs at 25°C. The results show that the rate-limiting step in the exchange of labeled water is not permeation through the cell membrane but diffusion in the bulk of the intracellular water.

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