THE PRODUCTION OF HUMAN A-BLOOD AND B-BLOOD GROUP LIKE SUBSTANCES BY INVITRO MAINTAINED SECOND STAGE TOXOCARA-CANIS LARVAE - THEIR PRESENCE ON THE OUTER LARVAL SURFACES AND IN THEIR EXCRETIONS SECRETIONS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 54  (3) , 625-633
Abstract
In vitro maintained 2nd stage T. canis larvae do not bind polyclonal anti-human A or B blood group antibodies onto their outer surface at 37.degree. C as detected by indirect fluorescence. When larvae are incubated at 2.degree. C under the same experimental conditions, intense fluorescence over the whole outer surface is observed. Reincubation of such larvae at 37.degree. C results in a gradual loss of surface fluorescence. This loss is complete after 3 h. The excretions/secretions (ES) of in vitro maintained larvae can neutralize the reactivity of polyclonal anti-human A and B typing antisera against their respective erythrocytic antigens. Rabbits infected with T. canis infective eggs or immunized with ES produce elevated isohemagglutinin titers against A and B erythrocyte antigens. The presence of epitopes, on the outer surfaces of T. canis larvae and in ES, which cross-react with human ABO blood group antibodies must be borne in mind when larvae or their products are used for the serodiagnosis of human toxocaral larva migrans.

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