QUANTITATIVE AMINO-ACID COMPOSITION AND CHARACTERISTICS OF AMINO-GROUPS OF RISTOMYCIN AND PRODUCTS OF ITS PARTIAL ACID-HYDROLYSIS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 23  (1) , 38-43
Abstract
The quantitative amino acid composition of ristomycin A, a glycopeptide antibiotic, peptides I-IV (from partial acid hydrolysis of the antibiotic) and their dinitrophenylic derivatives was determined. Ristomycin and free peptides I-IV contained 1 residue of ristomycinic acid and 1 residue of actinoidinic acid, diamino-dicarbonic amino acids of the glycylphenolic type. Peptides I-IV had close MW, i.e., 1100-1200 and differed from each other in the gradually increasing numbers of NH2- and COON-groups, from 1 in peptide I to 4 in peptide IV. The quantitative amino acid analysis of the dinitrophenylic derivatives of ristomycin and peptides I-IV showed that the free NH2-group in peptide I belonged to ristomycinic acid, the same as in the antibiotic, while in peptides III-IV at least 1 of the free NH2-groups belonged to ristomycinic acid and the other belonged to actinoidinic acid.

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