Partial Sequence Analysis of 130 Randomly Selected Maize cDNA Clones
Open Access
- 1 January 1993
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 101 (1) , 329-332
- https://doi.org/10.1104/pp.101.1.329
Abstract
As part of a project to identify novel maize (Zea mays L. cv B73) genes functionally, we have partially sequenced 130 randomly selected clones from a maize leaf cDNA library. Data base comparisons revealed seven previously sequenced maize cDNAs and 18 cDNAs with sequence similarity to related maize genes or to genes from other organisms. One hundred five cDNAs show little or no similarity to previously sequenced genes. Our results also establish the suitability of this library for large-scale sequencing in terms of its large insert size, proper insert orientation, and low duplication rate.Keywords
This publication has 10 references indexed in Scilit:
- Expressed genes, Alu repeats and polymorphisms in cosmids sequenced from chromosome 4p16.3Nature Genetics, 1992
- Introns in sequence tagsNature, 1992
- Caenorhabditis elegans expressed sequence tags identify gene families and potential disease gene homologuesNature Genetics, 1992
- A survey of expressed genes in Caenorhabditis elegansNature Genetics, 1992
- Automated DNA sequencing and analysis of 106 kilobases from human chromosome 19q13.3Nature Genetics, 1992
- The C. elegans genome sequencing project: a beginningNature, 1992
- Sequence identification of 2,375 human brain genesNature, 1992
- Isolation of a large number of novel mammalian genes by a differential cDNA library sreening strategyNucleic Acids Research, 1991
- Complementary DNA Sequencing: Expressed Sequence Tags and Human Genome ProjectScience, 1991
- Light-regulated gene expression during maize leaf development.The Journal of cell biology, 1984