Stability of N-Terminal Pro-Brain Natriuretic Peptide after Storage Frozen for One Year and after Multiple Freeze-Thaw Cycles

Abstract
Twenty-five samples were collected from healthy and heart failure clinic adult volunteers under informed consent according to the policies of the Washington University School of Medicine’s Institutional Review Board. All glass collection tubes were from Becton Dickinson. Lithium-heparin plasma (prod. no. 367686) was collected from all participants (n = 25). Serum was collected in either 10-mL Vacutainer® SST (prod. no. 366510) or 10-mL silicone-coated serum tubes (prod. no. 366430). EDTA plasma was collected in either 3-mL K2EDTA tubes (prod no. 367856,) or 7-mL K3EDTA tubes (prod. no 366450).