ISOLATION OF RIBOSOMES FROM CYSTS OF ENTAMOEBA INVADENS
- 1 January 1968
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 36 (1) , 45-51
- https://doi.org/10.1083/jcb.36.1.45
Abstract
A reproducible method for the preparation of 75S ribosomes from cysts of Entamoeba invadens is presented. The method depends on the inactivation of the cyst's ribonuclease by high levels of Bentonite. The ribosomes are found to be extremely sensitive to ribonuclease, and to be stabilized by the addition of manganous and calcium ions to the magnesium customarily employed. Reasons are given for equating these ribosomes with the particles of which the crystalline chromatoid bodies are made.Keywords
This publication has 1 reference indexed in Scilit:
- The visualization of polyribosomal structureJournal of Molecular Biology, 1963