SIMPLE MEANS OF OBTAINING CONCENTRATION OF INFLUENZA HEMAGGLUTINATING ANTIGEN

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Abstract
Allantoic-amniotic fluids, from influenza-infected chick embryos, were stored at 4[degree] to 6[degree]C between use as diagnostic influenza hemagglutinating antigens. Under these conditions the fluids yielded in a few days the customary white flocculent precipitate of urates and other embryonic products. It was found that the hemagglutinins became concentrated in the precipitate. The antigen could be concentrated further by 2 or 3 successive cold precipitations, but eventually the hemagglutinating titer differential between supernatant and precipitate became so small that further decantation of supernantant would have wasted antigen. The concentrated antigens were physically and anti-genically stable for as long as 9 months when stored at 4[degree] to 6[degree]C, and performed reliably as antigens in diagnostic hemagglutination-inhibition tests.

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