The spermosphere model. I. Its use in growing, counting, and isolating N2-fixing bacteria from the rhizosphere of rice
- 1 August 1982
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 28 (8) , 922-928
- https://doi.org/10.1139/m82-139
Abstract
The composition of the N2-fixing microflora of a rice rhizosphere was studied by a new enrichment and isolation procedure: rather than using an artificial C source, the exudates from a germinating seed ("the spermosphere model") are used. In this study, this system gave N2-fixing isolates with a frequency of 65%. Thirty-two of the many isolates obtained have been studied in detail; these were Klebsiella oxytoca, Enterobacter cloacae, Pseudomonas paucimobilis, and Azospirillum. All these bacteria were present at densities higher than 105 per gram of dry soil in the intial rice rhizosphere.This publication has 4 references indexed in Scilit:
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