PARTIAL-PURIFICATION AND CHARACTERIZATION OF A GROWTH-FACTOR FOR MACROPHAGE PROGENITOR CELLS WITH HIGH PROLIFERATIVE POTENTIAL IN MOUSE BONE-MARROW

  • 1 January 1982
    • journal article
    • research article
    • Vol. 60  (2) , 503-508
Abstract
A population of macrophage progenitor cells with high proliferative potential was recently demonstrated in post-fluorouracil-treated and normal mouse bone marrow (BM) in vitro, when a newly discovered growth factor (synergistic activity, SA) was combined with a macrophage colony-stimulating factor (CSF) as a proliferative stimulus. SA, present in human spleen (HSCM) and (HPCM), were studied and found to be unstable to trypsin digestion and to heating at .gtoreq. 50.degree. C, stable between pH 4-9, nonadherent to Con-A[concanavalin A]-Sepharose, and to have an isoelectric point between pH 5-5.8 and a MW of 14,000-21,000 as indicated by gel filtration chromatography. SA from both HSCM and HPCM were purified 89- and 122-fold, respectively, by precipitation of extraneous proteins at pH 5 followed by chromatographing twice on Sephacryl S200. Neither of these partially purified SA contain any CSF for mouse BM. The SA from HSCM and HPCM may be closely related and they are structurally different from CSF derived from various murine sources that are stable to proteolytic enzymes and heat.