COMPARISON OF ESTROGEN-RECEPTOR ENZYME-IMMUNOASSAY AND A RADIOLIGAND BINDING ASSAY (ISOELECTRIC-FOCUSING) IN ADENOCARCINOMA OF THE UTERINE BODY

  • 1 January 1987
    • journal article
    • research article
    • Vol. 34  (3) , 313-317
Abstract
Thirty-two samples from adenocarcinoma of the uterine body were analyzed for estrogen receptor (ER). ER was measured with two techniques: isoelectric focusing (IF) in polyacrylamide gels and enzyme immuno assay (EIA) with monoclonal antibodies. When comparing, a very good correlation between IF and EIA was found. ER recovery was, however, increased twice when using the latter technique. The advantages of EIA in terms of its rapidity and simplicity should be considered against its, at present, greater expenses compared with methods based on radiolabeled ligands.