Measurement of gliclazide in plasma by radial compression reversed-phase liquid chromatography.
Open Access
- 1 November 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 30 (11) , 1789-1791
- https://doi.org/10.1093/clinchem/30.11.1789
Abstract
We describe a rapid, specific, and precise analysis for gliclazide in plasma by radial compression, reversed-phase, "high-performance" liquid chromatography. Gliclazide and the internal standard, 3-chlorogliclazide, are eluted after 4.4 and 6.8 min, respectively. Only 100 microL of plasma and minimal sample workup are required. The limit of detection for gliclazide in plasma is 0.5 mg/L (1.55 mumol/L) at 229 nm. Precision (CV) of the assay for 10 and 1 mg of gliclazide per litre is 2.1% and 6.4%, respectively.This publication has 4 references indexed in Scilit:
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