Optimization of Solid Phase Extraction of Oxytetracycline from Fish Tissue and its Determination by HPLC

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Abstract
The extraction of oxytetracycline from fish tissues by use of solid phase extraction columns has been studied. The best recovery was obtained by using a C8 column and eluting with water/acetone (5% and 10% mixtures). The sensitivity of the method was 5 ng/g for muscle and 10 ng/g for liver. The repeatability of the assay on 10 samples at 1 μg/g and 0.1 μg/g was determined by the standard deviation equal to 4.2% and 6.7%, respectively. The recovery was 89.3% and 94.8%, respectively. The recovery of the internal standard (demeclocycline) was 82.8%.