ULTRAFILTRATION OF ANTERIOR PITUITARY KETOGENIC AND GROWTH PRINCIPLES

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Abstract
A neutralized alkaline extract of beef ant. pituitary was subjected to ultrafiltration through graded cellophane membranes. The pore sizes employed were 1.6 m[mu], 3.3 m[mu], 5.6 m[mu], and 6.9 m[mu]. The smallest size filter passed no protein and no ketogenic or growth principle. The 3.3 m[mu] filter passed a trace of protein and the filtrate contained both ketogenic and growth activity. Ketogenic and growth principles therefore appear to be composed of molecules comparable in size to small molecular proteins. At no pore size was there any appreciable selective conc. of the above 2 ant. pituitary principles.