A novel experiment for the quantitative measurement of CSA(1HN)/CSA(15N) cross-correlated relaxation in 15N-labeled proteins*
- 1 January 2000
- journal article
- Published by Springer Nature in Journal of Biomolecular NMR
- Vol. 16 (2) , 171-174
- https://doi.org/10.1023/a:1008347012429
Abstract
An experiment is presented which allows for the quantitative measurement of the relaxation interference between the 1HN CSA and 15N CSA interactions in 15N labeled proteins. A constant-time buildup scheme is used to measure the differential relaxation rate, η, between double-quantum (DQ) and zero-quantum (ZQ) 1HN-15N coherences. The CSA/CSA experiment was recorded at three different Bo field strengths. The CSA(1HN)/CSA(15N) cross-correlation rate was obtained from the linear fit of the measured rate, η, versus Bo2 for 77 residues of the EH2 domain from mouse Eps15.Keywords
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