Fluid phase and mannose receptor‐mediated uptake of horseradish peroxidase in mouse bone marrow‐derived macrophages. Biochemical and ultrastructural study

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Abstract
A detailed study of horseradish peroxidase (HRP) uptake by in vitro cultured bone marrow-derived macrophages was undertaken. Biochemical quantitations performed over a wide range of HRP concentrations, in the absence or presence of yeast mannan, showed that these macrophages pinocytose HRP by both fluid phase and mannose receptor-mediated uptake. The relative contribution of these 2 types of endocytosis varied with the concentration of enzyme in the extracellular medium. A morphological study at the light and EM levels conducted in parallel confirmed the biochemical data.