Effect of inclusion body production on culture turbidity and cell dry weight in growing bacterial cultures
- 1 January 1990
- journal article
- research article
- Published by Wiley in Biotechnology Progress
- Vol. 6 (1) , 48-50
- https://doi.org/10.1021/bp00001a007
Abstract
An approach to the optimization of product yield in an inducible inclusion body-producing system is presented. Following induction by indoleacrylic acid (IAA) of a trpLE-HIVgp41 fusion protein, we found a large increase in culture turbidity and single-cell dry weight. After an initial transition phase, new and constant values for specific growth rate, single-cell light turbidity, and single cell dry weight were achieved, allowing for the determination of optimal conditions for product formation.This publication has 0 references indexed in Scilit: