Use of an In Vivo Biotinylated Single-Chain Antibody as Capture Reagent in an Immunometric Assay to Decrease the Incidence of Interference from Heterophilic Antibodies
Open Access
- 1 May 2005
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 51 (5) , 830-838
- https://doi.org/10.1373/clinchem.2004.046979
Abstract
Background: Heterophilic antibodies are a common source of interference in immunometric assays. We tested the hypothesis that the incidence of such interference could be decreased by use of a recombinant in vivo-biotinylated single-chain antibody (scFv) as the capture reagent.Keywords
This publication has 46 references indexed in Scilit:
- The War on Heterophilic Antibody InterferenceClinical Chemistry, 2005
- Production of A Single Chain Anti-CEA Antibody From the Hybridoma Cell Line T84.66 Using a Modified Colony-Lift Selection Procedure to Detect Antigen-Positive ScFv Bacterial ClonesHybridoma, 1998
- Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display systemJournal of Immunological Methods, 1997
- Reducing interference from heterophilic antibodies in a two-site immunoassay for carcinoembryonic antigen (CEA) by using a human/mouse chimeric antibody to CEA as the tracerJournal of Immunological Methods, 1995
- Use of Peptide Libraries to Map the Substrate Specificity of a Peptide-Modifying Enzyme: A 13 Residue Consensus Peptide Specifies Biotinylation in Escherichia coliNature Biotechnology, 1993
- Selection of monoclonal antibodies for use in an immunometric assay for carcinoembryonic antigenJournal of Immunological Methods, 1990
- Interference of IgM rheumatoid factor with nephelometric C-reactive protein determinationsJournal of Immunological Methods, 1985
- Population Study of Heterophile AntibodiesVox Sanguinis, 1980
- CIRCULATING ANTIBODIES TO OVINE AND BOVINE IMMUNOGLOBULIN IN HEALTHY SUBJECTS: A HAZARD FOR IMMUNOASSAYSThe Lancet, 1980
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976