Gel Permeation for the On Line Removal of Proteins from Plasma Samples Prior to HPLC
- 1 June 1986
- journal article
- research article
- Published by Taylor & Francis in International Journal of Environmental Analytical Chemistry
- Vol. 25 (1) , 209-220
- https://doi.org/10.1080/03067318608077089
Abstract
Gel permeation chromatography (GPC) has been investigated for the on line removal of proteins from plasma samples prior to their analysis by HPLC. The results show that GPC is a mild and effective way to remove proteins from plasma samples. It can very well be coupled on line to HPLC, providing the solutes are suitable for preconcentration on the analytical column itself or on a small pre-column, after the GPC. Under these conditions excellent reproducibility and accuracy can be obtained.Keywords
This publication has 6 references indexed in Scilit:
- Solid-surface sample handling techniques in organic trace analysisTrAC Trends in Analytical Chemistry, 1981
- Automated on-line multi-dimensional high-performance liquid chromatographic techniques for the clean-up and analysis of water-soluble samplesJournal of Chromatography A, 1981
- Application of on-column concentration of deproteinized serum to the HPLC-determination of anticonvulsantsChromatographia, 1980
- The use of a pre-column for the direct high-performance liquid chromatographic determination of the anti-depressants clovoxamine and fluvoxamine in plasmaJournal of Chromatography B: Biomedical Sciences and Applications, 1980
- Two-dimensional column liquid chromatographic technique for resolution of complex mixturesJournal of Chromatography A, 1978
- Coupled column chromatography employing exclusion and a reversed phaseJournal of Chromatography A, 1978