Immunohisto-cytochemical localization of glutathione-peroxidase (GSH-PO) in the rat testis.

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Abstract
Immunohisto-cytochemical localization and biochemical activity of glutathione-peroxidase (GSH-PO) in testes were studied in normal untreated rats; 1 wk after hypophysectomy; and gonadotropin administration after hypophysectomy, in order to clarify the role of GSH-PO. In the normal untreated group, GSH-PO was immunohisto-cytochemically localized in cytoplasm in close relation with the smooth endoplasmic reticulum of Leydig cells. In the hypophysectomized group, the Leydig cells were markedly atrophic and GSH-PO was localized in the cytoplasm around the swollen mitochondria or vesiculated smooth endoplasmic reticulum. By gonadotropin administration to the hypophysectomized group, the Leydig cells became markedly hypertrophic and contained well developed smooth endoplasmic reticulum. Immunohisto cytochemically, GSH-PO was observed in cytoplasm near the well developed smooth endoplasmic reticulum of the Leydig cells. Intensity of GSH-PO staining was stronger than that of the other experimental group. The biochemical GSH-PO activity of the isolated Leydig cells was also increased as compared to the other groups. There is apparently a very close relationship between the testicular GSH-PO and membrane metabolism including reduction of lipid peroxides of smooth endoplasmic reticulum.