Dynamic Properties of Protein and Water Studied Through Effects of Water Content on Water Relaxations in Erythrocytes
- 1 November 1994
- journal article
- Published by IOP Publishing in Japanese Journal of Applied Physics
- Vol. 33 (11R)
- https://doi.org/10.1143/jjap.33.6417
Abstract
Magnetizations of water protons in human erythrocytes were measured using an off-resonance irradiation technique by varying the irradiation frequency and water content. The frequency dependences of the magnetizations were well expressed by the rate equations, which gave the tumbling time of hemoglobin protons and the intrinsic relaxation rates of water and hemoglobin protons. Analysis of the data determined the amounts and the average correlation times of bound water fractions. The average lifetime of the bound water in the interior of the protein with respect to exchange with the bulk water was found to be 1.0±0.3 µ s. The estimation of the surface hydration layer showed excellent agreement with the amount measured by thermodynamical methods.Keywords
This publication has 23 references indexed in Scilit:
- Polypeptide hydration in mixed solvents at low temperaturesJournal of the American Chemical Society, 1992
- Proton exchange with internal water molecules in the protein BPTI in aqueous solutionJournal of the American Chemical Society, 1991
- Studies of protein hydration in aqueous solution by direct NMR observation of individual protein-bound water moleculesJournal of the American Chemical Society, 1989
- Water of hydration in the intra- and extra-cellular environment of human erythrocytesBiochemistry and Cell Biology, 1988
- NMR relaxation of protein and water protons in diamagnetic hemoglobin solutionsBiochemistry, 1985
- NMR hybrid relaxation methods of studying chemical, physical, and spin exchange. I. General theory and experimental methodsJournal of Magnetic Resonance (1969), 1980
- The measurement of cross-relaxation effects in the proton NMR spin-lattice relaxation of water in biological systems: Hydrated collagen and muscleJournal of Magnetic Resonance (1969), 1978
- Water proton magnetic resonance studies of normal and sickle erythrocytes Temperature and volume dependenceBiochimica et Biophysica Acta (BBA) - General Subjects, 1976
- CURRENT STATUS OF THE WATER-STRUCTURE PROBLEM; APPLICATION TO PROTEINSAnnals of the New York Academy of Sciences, 1973
- Zur Theorie der kernmagnetischen Relaxation in ZweiphasensystemenAnnalen der Physik, 1965