Abstract
A hypotonic treatment using a low osmolal solution (0.038 Os/kg) led to a poor staining of the W chromosome in all our investigated bird species (chicken, turkey, pheasant, goose, pigeon, bearded vulture, and barn owl). A turkey establishing a ZWW karyotype was detected.Key words: birds, W chromosome, differential Giemsa staining, ZWW karyotype.

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