Efficiency of Particle-Bombardment-Mediated Transformation Is Influenced by Cell Cycle Stage in Synchronized Cultured Cells of Tobacco

Open Access

1 December 1991

journal article
Published by Oxford University Press (OUP) in Plant Physiology

Vol. 97 (4) , 1585-1587
https://doi.org/10.1104/pp.97.4.1585

Abstract

Plasmid DNA pB1221 harboring β-glucuronidase gene was delivered to synchronized cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells of different cell cycle stages by a pneumatic particle gun. The cells bombarded at M and G₂ phases gave 4 to 6 times higher transformation efficiency than those bombarded at the S and G₁ phases.

Keywords

TOBACCO
PNEUMATIC
GUN
CULTURED CELLS
YELLOW
GAVE
BOMBARDED
BRIGHT
TABACUM

This publication has 1 reference indexed in Scilit:

Evidence That More than 90% of β-Glucuronidase-Expressing Cells after Particle Bombardment Directly Receive the Foreign Gene in their Nucleus
Plant Physiology, 1991