Radioimmunoassay for the Quantitative Determination of Quinine in Cultured Plant Tissues

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Abstract
The development of a radioimmunossay for the determination of pieogram amounts of quinine [an antimalarial and a bittering agent used by the soft-drinks industry] is described. The antiserum, raised against a conjugate of quinine and bovine serum albumin, is highly specific to the Cinchona quinoline alkaloids having the 8S configuration and will not cross-react to any detectable extent with the 8R series. Neither will it cross-react with quininone or cinchonidinone, the common 9-keto precursors. Using this assay a number of clones of C. ledgeriana were screened for their quinine content.