Analysis of mutagenic DNA repair in a thermoconditional mutant of Saccharomyces cerevisiae
- 1 January 1986
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 202 (1) , 68-74
- https://doi.org/10.1007/bf00330519
Abstract
Recent studies regarding the influence of cycloheximide on the temperature-dependent increase in survival and mutation frequencies of a thermoconditional rev2 mutant lead to the suggestion that the REV2-coded mutagenic repair function is UV-inducible. In the present study we show that stationary-phase rev2 ts cells are characterized by a biphasic linear-quadratic dose-dependence of mutation induction (“mutation kinetics”) of ochre alleles at 23° C (permissive temperature) but linear kinetics at the restrictive temperature of 36° C. Mathematical analysis using a model based on Poisson statistics and a further mathematical procedure, the calculation of “apparent survival”, support the assumption that the quadratic component of the reverse mutation kinetics investigated can be attributed to a UV-inducible component of mutagenic DNA repair controlled by the REV2 gene.This publication has 43 references indexed in Scilit:
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