Influence of Ammonia and of Urea Upon L. Acidophilus 4646

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Abstract
Glycolysis of washed-cell suspensions of Lactobacillus acidophilus 4646 (recently reclassified as L. casei) was studied by Warburg technic, pH 7.4, temp. 38[degree]C. Phosphate buffers depressed glycolytic activity in log. proportion to their concn. NH4 was no more effective than K phosphate. Urea, 4 [image], inhibited completely; lesser concns. of urea were less effective. Preincubation of washed cells with 4 [image] urea for 30 mins. reduced the subsequent glycolytic rate. No urease activity could be demonstrated in these cells, using a modification of Conway''s technic in which Warburg flasks served as diffusion vessels, alkali was added from side-arm, and NH3 was diffused at 38[degree]C for 16 hrs.