Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene
- 1 January 1989
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 171 (1) , 577-580
- https://doi.org/10.1128/jb.171.1.577-580.1989
Abstract
The Escherichia coli K-12 ackA gene, which encodes an acetate kinase, was cloned. The acetate kinase activities of ackA+ plasmid-containing strains were amplified 160- to 180-fold. The complete nucleotide sequence of the ackA gene was determined. It was deduced that the ackA gene coded for a protein of 400 amino acids with an Mr of 43,297. The ackA gene was found to be located about 15 kilobases upstream of the purF-folC-hisT region of the chromosome.This publication has 37 references indexed in Scilit:
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