Co-localization of SV40 T antigen and p53 with immunological analogues of microtubule-associated protein-1 on the nuclear skeleton.

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Abstract
Rat SV-3Y1 cells were stained with double immunofluores-cence. Treatment of cells with detergent and salt removed about 80 % of the antigens and revealed immunofluorescent flecks of the SV40 large T antigen and p53 bound to the nuclear skeleton. These flecks exactly corresponded to the fluorescent spots produced by antibodies against microtubule associated protein-1. The MAP-1 analogues may function in the initiation of DNA synthesis through the interaction with T-antigen and p53.