PEPSIN: A NEW EXTRACT ANT FOR SERUM THYROXINE BY ENZYMATIC DIGESTION OF THYROXINE BINDING PROTEINS
- 1 January 1976
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 42 (1) , 189-192
- https://doi.org/10.1210/jcem-42-1-189
Abstract
The use of a pepsin solution to digest serum proteins results in quantitative release of thyroxine from carrier proteins. This extraction method greatly simplifies the original Murphy and Pattee (1) competitive protein-binding assay for serum thyroxine. The simplicity and efficiency of this extractant suggest that it may have application in radioimmunoassay and competitive protein-binding assay for nonpeptide ligands in serum.Keywords
This publication has 1 reference indexed in Scilit:
- The Denaturation of Pepsin. II. Hydrogen Ion Equilibria of Native and Denatured Pepsin1Journal of the American Chemical Society, 1958