Mapping the transcription site of the SV40-specific late 16 S mRNA
- 1 October 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (10) , 1995-2006
- https://doi.org/10.1093/nar/2.10.1995
Abstract
This paper describes the purification of polysomal RNA from SV4O-lytically infected CVl (monkey) cells and separation of the two distinct classes of SV4O-specific mRNA sedimenting at 16 S and 19 S. These classes have been hybridized with the whole SV4O DNA genome as well as with the SV40 Hind fragments. The results have permitted the mapping of SV40-specific late 16 S mRNA from approximately 0.945 to 0.175 map units.Keywords
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