Single-Radial-Hemolysis Test for the Assay of Rubella Antibody in Antenatal, Vaccinated, and Rubella Virus-Infected Patients

Publisher Website
Google Scholar
Abstract
The single radial hemolysis test (SRHT) is a simple, convenient and sensitive method that provides accurate measurement of Ig[immunoglobulin]G antibody [Ab] to rubella virus. Lipoprotein inhibitors of rubella hemagglutination [HA] do not interfere with the SRHT; the assay of low levels of Ab is more reliable by SRHT than by the rubella HA inhibition test (RHIT). The technique for the SRHT in a form suitable for large numbers of tests is detailed and compared with that of the RHIT and the reproducibility of the SRHT in routine operation is assessed. Results obtained by testing various populations in western Australia are used to show the application of the SRHT in the diagnosis of rubella and in the monitoring of rubella vaccination programs.