The Interaction Between Bovine Ribonuclease and Antibody: A Study of the Mechanism of Enzyme Inhibition by Antibody

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Abstract
Summary: It has been shown that the inhibitory capacity of antibody obtained in the course of immunization, first subcutaneously in adjuvant and then by repeated intravenous injections, varies in the course of immunization and that there is a progression towards completely inhibitory antibody. The degree of inhibition exercised for a given antibody varies with the sequence of molecular weights of products; thus the inhibitory capacity is greatest in the reaction of enzyme on nucleic acid, is smallest in the inhibition of the formation of 3′ cytidylic acid, and is intermediate with respect to the formation of cyclic dicytidylic acid. The effect of antibody is identical in the inhibition of two very different reactions, resultng in the formation of products of similar molecular weight, 3′ cytidylic acid and the methyl ester of 3′ cytidylic acid. The above observations are best explained by the assumption that the inhibitory capacity of antibody depends on a mechanism of steric hindrance.

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