Scanning Electron Microscopy of Opaque and Transparent States in Reversible Calf Lens Cataracts

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Abstract
The microstructure of transparent and opaque calf lenses was studied using scanning electron microscopy. Cells in opaque lenses are very similar to cells in transparent lenses. Opacification [apparently] does not require major changes in the structure or relative arrangement of the lens cells. Numerous, characteristic flat patches on the membrane surfaces of cells in opaque lenses were observed. Laser diffraction and measurement of light transmittance established that glutaraldehyde fixation does not alter the optical properties or the cellular organization of the lens tissue.