A Simple Procedure to Obtain One-Micrometer Sections of Routinely Embedded Paraffin Material
- 1 January 1989
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 64 (1) , 39-42
- https://doi.org/10.3109/10520298909108041
Abstract
By freezing blocks of paraffin-embedded tissues to a convenient temperature it is possible to obtain routinely 1 µm sections that can be further processed as normal thicker sections. Normal and disposable steel knives can be used and the staining time should be increased in most procedures. Gradual freezing of blocks to the temperature of dry ice is the simplest and safest way to obtain an adequate temperature. The best results were obtained using as fixative 4% paraformaldehyde in phosphate buffered saline solution.Keywords
This publication has 1 reference indexed in Scilit:
- A Procedure for Cutting One-Micrometer Sections of Wax Embedded SkinStain Technology, 1984