Immunocytochemical Localization of Hydroxyproline-Rich Glycoproteins Accumulating in Melon and Bean at Sites of Resistance to Bacteria and Fungi

Publisher Website
Google Scholar
Abstract
Immunocytochemistry was used to locate hydroxyproline-rich glycoproteins (HRGPs) during incompatible interactions in hypocotyls of Phaseolus vulgaris inoculated with Colletotrichum lindemuthianum, and in leaves of Phaseolus vulgaris and Cucumis melo inoculated with Pseudomonas syringae pv. phaseolicola or with P. fluorescens (a saprophyte). Postembedding immunogold labeling with antibodies to melon HRGP2b showed that HRGPs accumulated in walls of living plant cells adjoining dead hypersensitive cells during resistant reactions to both the fungus and bacteria. HRGPs were also detected in papillae encasing intracellular fungal hyphae and in small papillae adjacent to bacterial colonies. Intercellular material encapsulating cells of P. fluorescens also contained HRGPs. The highly localized accumulation of HRGPs at sites where the growth of bacteria and fungi is arrested supports the involvement of these structural glycoproteins in disease resistance.

This publication has 0 references indexed in Scilit: