Unusual long terminal repeat sequence of a retrovirus transmissible mouse (VL 30) genetic element: identification of functional domains
- 25 April 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (8) , 3445-3460
- https://doi.org/10.1093/nar/12.8.3445
Abstract
We have determined the nucleotide sequence and mapped the tranacriptional boundaries in the long terminal repeats (LTRs) and adjacent regions of a retrovirus transmisaible virus-like 30S (VL30) mouse genetic element. The 572 base pair LTRs contain transcriptional regulatory sequences and are bounded by short imperfect repeats, with a minus strand tRNA gIy primer binding site and a purine rich plus strand primer site flanking each of their inner boundaries. The 3′ end of each LTR consists of an extensive 80 base pair redundancy of tRNA primer site and inverted repeat sequences while 41 and 47 base pair imperfect tandem repeats are present between the 5′ capping site and the putative polyadenylation signal. Comparison with other retrovirus-like LTR sequences suggests possible modes of recmbination that could occur between VL30 and other genetic elements.Keywords
This publication has 31 references indexed in Scilit:
- A novel approach to cloning transcriptionally active retrovirus-like genetic elements from mouse cellsNucleic Acids Research, 1983
- Heterogeneity of “virus-like” genes encoding retrovirus-associated 30S RNA and their organization within the mouse genomeCell, 1980
- Definition of the 5′ and 3′ ends of transcripts of the ovalbumin geneCell, 1980
- Assumed initiation site of fibroin gene transcription.Proceedings of the National Academy of Sciences, 1979
- A detailed model of reverse transcription and tests of crucial aspectsCell, 1979
- The nucleotide sequen of human tRNAGly(anticodon GCC)Nucleic Acids Research, 1979
- Analysis of a 5′ leader sequence on murine leukemia virus 21S RNA: Heteroduplex mapping with long reverse transcriptase productsCell, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- RNA metabolism of murine leukemia virus. III. Identification and quantitation of endogenous virus-specific mRNA in the uninfected BALB/c cell line JLS-V9Journal of Virology, 1976