NITRATE REDUCTION IN AEROBIC BACTERIA AND THAT IN ESCHERICHIA COLI COUPLED TO PHOSPHORYLATION

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Abstract
Among aerobic bacteria, Serratia marcescens and Pseudomonas fluorescens liquefaciens can grow anaerobically in nitrate-containing broth medium, whereas 2 strains of Bacillus subtilis (P.C.L. 219, and N.R.R.L. 558), and B. mycoides Krai, B. mycoides roseus and Ps. fluorescens N.R.R.L. grow only aerobically in the same medium. During growth, a fairly large amount of nitrite is produced. Resting cells of S. marcescens reduce nitrate to nitrite in the presence of glucose or leucomethylene blue (MB.H2), whereas those of Pseudomonas carry it out by utilization of formate but not MB.H2- The nitrate-reducing activity of these bacteria is inhibited by 0.001M KCN, NaN3, and slightly by 0.01M alpha, alpha''-dipyridyl;0.014[image] versene and 1 atm. CO induces a slight inhibition (10%) only for Pseudomonas. Resting cells of E. coli exhibit nitrate reduction together with increased p32-turnover in the presence of both nitrate and fumarate; but the latter phenomenon does not take place in the presence of either nitrate or fumarate alone.

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