Parallel Stimulation of Sugar Transport and Glycogen Formation by a Synthetic Insulin-Dextran Complex in Diaphragms

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Abstract
The effects of a soluble insulin—dextran complex on the distribution of l-14C—L—arabinose and on the incorporation of U—14C—D—glucose into glycogen were examined in rat “intact” hemidiaphragm preparations. The complex was prepared by coupling crystalline bovine insulin covalently with dextran (av mol wt, 40,000). The insulin—dextran caused the acceleration of glycogen formation in parallel with the facilitation of sugar transport in quite a similar manner to that of native insulin, although insulin—dextran was to some extent less potent than native insulin in both of the above actions. The present results indicate that insulin exerts its effects on glycogenesis under conditions precluding the entry of the hormone into the cell. The binding of insulin with the cell membrane structures not only stimulates transmembrane sugar transport but initiates a propagation of events by which glycogen formation system is directly stimulated within the cell. (Endocrinology91: 1442, 1972)

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