Isolation and amino acid sequence of a novel 6.8-kDa mitochondrial proteolipid from beef heart
- 15 January 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 260 (1) , 122-126
- https://doi.org/10.1016/0014-5793(90)80082-t
Abstract
We have isolated a 6.8 kDa proteolipid from an acidic chloroform/methanol extract of bovine cardiac muscle. The molecular mass of the polypeptide was measured by fast atom bombardment-mass spectrometry (FAB-MS) (m/z 6834.1). Its amino acid sequence was partly determined by direct sequencing and completed by characterization of cyanogen bromide and tryptic fragments (sequencing, FAB-MS and amino acid analysis). The polypeptide consists of 60 amino acid residues. Polyclonal antibodies raised in rabbit allowed its localization by electroimmunoblotting in mitochondriaKeywords
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