The Adaptation of a Standard Curve to the Turbidometric Method of Assay of Hyaluronidase in Bull Semen

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Abstract
In using the turbidometric assay for hyaluronidase, considerable flexibility in the calculation of hyaluronidase potency in semen is achieved by reference to a standard prepn. of hyaluronidase. The coeff. of correlation between mg. of standard hyaluronidase and colorimeter meter reading was -0.985 [plus or minus] 0.006. Hyaluronidase assay values of semen in terms of mg. of standard hyaluronidase, obtained by diluting semen at various rates, conformed to the standard regression line of purified hyaluronidase. A coeff. of correlation of +0.954 [plus or minus] 0.008 was obtained between turbidity reducing units and mg. of standard hyaluronidase equivalent for 117 semen samples, indicating the essential sameness of the 2 measures of hyaluronidase potency. The results indicate the validity of expressing semen hyaluronidase potencies in terms of mg. of a standard hyaluronidase preparation.