A selective medium for enumerating low populations ofThielaviopsis basicolain tobacco field soils

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Abstract
A selective medium (TB-CEN) is described for the quantitative isolation of Thielaviopsis basicola from naturally infested tobacco field soils containing low population densities. The medium contains etridiazol and nystatin as selective inhibitors of undesired fungi, and unautoclaved carrot extract for selective enhancement. The pour-plate technique, along with incubation at 16-22.degree. C, yielded the greatest recovery of T. basicola on TB-CEN. The selective medium was as sensitive as carrot-disks, and better than three other selective media, for isolating T. basicola from tobacco field soils.