Tobacco Budworm: Development of a Spray Adjuvant to Increase Effectiveness of a Nuclear Polyhedrosis Virus123

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Abstract
At least 75% of responding neonate larvae of Heliothis virescens (F.) demonstrated a preferential feeding response to water extracts of cotton seed and corn silks, kernels and seed, and crude cottonseed oil, cottonseed flours, and soy flour. A cottonseed flour, the most preferred, was a likely candidate as one feeding stimulant in a microbial spray adjuvant. Exposing neonate larvae for 2 h to greenhouse cotton plants treated with a spray of a nuclear polyhedrosis virus from Autographa californica (Speyer) formulated with cottonseed flour, crude cottonseed oil, and sucrose produced 93.3% mortality due to viral infection after 10 days. Mortality was 33.3% among larvae exposed to plants treated with virus in water alone.

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