Mutations That Alter the Transmission of Chloroplast Genes in Chlamydomonas

Abstract
Two mutations are described that alter the pattern of inheritance of chloroplast genes in Chlamydomonas . The mutant gene mat-1 linked to the mating type allele mt - greatly increases the frequency of exceptional zygotes, i.e., zygotes that transmit chloroplast genes from the mt - (male) parent. In some crosses, 80-90% of the zygotes are biparental, transmitting chloroplast genes from both parents. The mat-2 mutation, linked to mt + , acts to decrease the frequency of exceptional zygotes below the spontaneous level. The mutant effects are discussed in terms of a DNA modification-restriction system, postulated to regulate the transmission of chloroplast DNA in zygotes.

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