A Trypsin Method for Coating Human Red Blood Cells with Plasma C3 and/or C4

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Abstract
Simple procedures are described for the production of human red blood cells [RBC] coated with C3 [complement component 3] and/or C4 by brief trypsin treatment of cell-plasma mixtures at room temperature. These cells (''EC3/4''-try) were unusual in that their agglutinability by anticomplement sera, as compared to sucrose-water RBC, was similar when tested by AutoAnalyzer but was markedly reduced in manual testing, especially against anti-C4. Both ''EC34''-try and ''EC3''-try were suitable indicator cells for determining the adequacy of anti-C3c and/or anti-C3d activities in reagents used for manual antiglobulin tests.