Culture of in vitro fertilized rabbit ova

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Abstract
Embryonic development of in‐vitro fertilized rabbit ova was assessed following in‐vitro culture in four different serum supplemented media. A mixture of Basal Medium Eagle (BME) and Ham's F10 medium (1:1) provided better support for in‐vitro development than Ham's F10, BME, or regular acidic saline (RAS). In‐vitro embryonic development in the BME/Ham's F10 mixture was synchronous with in‐vivo development through at least 55 hr of culture. After 54 hr of culture, embryos transferred to the oviduct of a synchronous pseudopregnant recipient were able to implant at the same rate as simultaneously transferred embryos grown in vivo. BME/Ham's F10 supplemented with 10% newborn calf serum was highly supportive of rabbit embryo development following in‐vitro fertilization.