cDNA‐derived amino‐acid sequence of lentil seedlings' amine oxidase

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Abstract
Lentil seedlings' amino oxidase (LSAO) cDNAs were identified in a cDNA λgi 10 library by plaques hybridization. The nucleotide sequence of a 2111 bp clone was determined. It contains part of a signal peptide, the complete sequence coding for the mature protein and the 3′‐untranslated region of the mRNA. The deduced protein sequence shows that the mature protein is composed of 569 amino acids with a molecular mass of 67 kda, also taking into account the glucidic component. The LSAOcDNA was identified by sequencing the N‐terminal part of the protein and several tryptic peptides. The protein sequence shows a characteristic hexapeptide present in amine oxidases containing 6‐hydroxydopa as the organic cofactor. Three conserved histidines might be the ligands of copper bound to the enzyme.